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Bits and Bugs | Applying bioinformatics to public health microbiology

Applying bioinformatics to public health microbiology

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Bits and Bugs | Applying bioinformatics to public health microbiology | bitsandbugs.org Reviews

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Applying bioinformatics to public health microbiology

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Using GATK to call indels in bacterial genomes | Bits and Bugs

https://bitsandbugs.org/2015/04/23/using-gatk-to-call-indels-in-bacterial-genomes

Applying bioinformatics to public health microbiology. Using GATK to call indels in bacterial genomes. April 23, 2015. If you are interested in calling indels with GATK, check out the below. If not, don’t. 8212;—————————————————–. 1 Have a sorted bam file. 2 Run GATK (v.2.6.5 in my case), with the following command. Java -Xmx4g -jar GenomeAnalysisTK.jar -T UnifiedGenotyper -nt 1 -R reference.fa -I sorted.bam -o indel.vcf -glm INDELS. 2a I investigated the GATK realigner around indels. As these will be ca...

2

PhD viva advice | Bits and Bugs

https://bitsandbugs.org/2014/12/30/phd-viva-advice

Applying bioinformatics to public health microbiology. December 30, 2014. I was just asked for some advice on PhD viva, so have turned the email into a blog post. 8212;—————. They often start by asking you to place your work into context of existing literature and summarise your main findings in 5-10 minutes, so have your summary ready. In terms of new articles, I only discussed ones that were directly relevant to my work, they were happy with that. As long as you seem to be aware of other organisms ...

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Thoughts on bioinformatics as wet-lab kits | Bits and Bugs

https://bitsandbugs.org/2014/11/02/thoughts-on-bioinformatics-as-wet-lab-kits

Applying bioinformatics to public health microbiology. Thoughts on bioinformatics as wet-lab kits. November 2, 2014. I have just read Sean Eddy’s thought provoking blog post. On the problems with how biology is handling high throughput sequencing which you should definitely read. Nick Loman tweeted an excerpt from Sean’s blog post saying something along the lines of ‘bioinformatics should be more like kits’, I have to admit my first thought was ‘ugh, no! 1) Tool installation. Bioinformaticians often ...

4

December | 2014 | Bits and Bugs

https://bitsandbugs.org/2014/12

Applying bioinformatics to public health microbiology. Monthly Archives: December 2014. I was just asked for some advice on PhD viva, so have turned the email into a blog post. —————— They often start by asking you to place your work into context of existing literature and summarise your main findings in 5-10 minutes, so have your summary ready. The main prep I did was to…. December 30, 2014. December 30, 2014. Lighter: better, faster, longer? December 11, 2014. Lauren Cowley is currently in Sierra Leone...

5

November | 2014 | Bits and Bugs

https://bitsandbugs.org/2014/11

Applying bioinformatics to public health microbiology. Monthly Archives: November 2014. Annotating phylogenetic trees with gene presence/absence etc. As a bioinformatician, I spend a lot of time generating, interpreting and presenting trees. One of the most powerful ways to get some added value out of a tree is to be able to compare the phylogeny with the accessory gene content, to see if there is something going on with the mobile genetic elements that…. November 4, 2014. November 2, 2014.

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LANGMEAD LAB @ JHU. LANGMEAD LAB @ JHU. You are free to use these materials. If you do, please sign the guestbook at the bottom of this page and say briefly how you’re using them. Algorithms for DNA Sequencing. 55 lectures, about 7 minutes each). Suffix tries and suffix trees. Lecture notes and IPython/Jupyter notebooks. What are Genomics and Computational Genomics? Strings and exact matching. Notebook: Strings in Python. Notebook: Naive exact matching. Notebook: Naive approximate matching. Genes and gen...

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Bits and Bugs | Applying bioinformatics to public health microbiology

Applying bioinformatics to public health microbiology. Programatically accessing internal nodes of a phylogeny. This post belongs in the ‘write it down before you forget’ category. Edit – this approach is necessary for handling the trees produced by RAxML, but not those produced by IQ-TREE. Edit #2 – Ancestral state reconstruction in RAxML does not seem to work very well. I’m trying IQ-TREE (v1.6) instead. I have a tree…. Continue reading →. Salmonella genomic epidemiology exercise. Trimmomatic: A flexib...

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