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microscopy is light at work - Introduction to LSFM

ernstelzer - microscopy is light at work

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microscopy is light at work - Introduction to LSFM | lightmicroscopy.de Reviews
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ernstelzer - microscopy is light at work
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1 SPIM
2 SPIM-SI
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6 multi-view
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8 LSFM
9 4Pi
10 confocal
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microscopy is light at work - Introduction to LSFM | lightmicroscopy.de Reviews

https://lightmicroscopy.de

ernstelzer - microscopy is light at work

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1

microscopy is light at work - Impressum

http://www.lightmicroscopy.de/pageID_6471543.html

Dr Ernst H.K. Stelzer. Tel 49 (6226) 91014.

2

microscopy is light at work - Ernst H.K. Stelzer

http://www.lightmicroscopy.de/pageID_6471537.html

Dr Stelzer started working on confocal fluorescence microscopy in 1983, developed the 4Pi microscope in the early 1990s and shortly afterwards introduced orthogonal and multi-lens detection schemes with theta microscopy. The latter work lead to the development of light-sheet-based fluorescence microscopy. Other contributions include the optical tweezers based photonic force microscope (PFM) and a novel and very successful approach to laser baser cutting devices. Dr Stelzer has been an organiser of about ...

3

microscopy is light at work - Projects

http://www.lightmicroscopy.de/pageID_6471540.html

Past and current projects. Dr Stelzer's group develops and applies instruments as wells as specimen preparation techniques that allow scientists to observe as well as to manipulate biological specimens efficiently and with high precision or high resolution. Since the early 1990s his long-term interest is to provide a complete set of tools that foster research in the life sciences under physiologically relevant conditions.

4

microscopy is light at work - Contact

http://www.lightmicroscopy.de/pageID_6471541.html

Dr Ernst H.K. Stelzer. European Molecular Biology Laboratory (EMBL). Cell Biology and Biophysics Unit (CBBU). D-69117 Heidelberg, Germany. Tel 49 (6221) 387 8 354. Fax 49 (6221) 387 98 354. Mobile 49 (170) 6357 168. Dr Ernst H.K. Stelzer. Tel 49 (6226) 91014.

5

microscopy is light at work - Publications

http://www.lightmicroscopy.de/pageID_6471539.html

Keller PJ, Pampaloni F, Lattanzi G, Stelzer EHK. 2008) Three-dimensional microtubule behaviour in Xenopus. Egg extracts reveals four dynamic states and state-dependent elastic properties. Biophys J,. Keller PJ, Pampaloni F, Stelzer EHK. 2007) Three-dimensional preparation and imaging reveal intrinsic microtubule properties, Nat Methods. Pampaloni F, Reynaud EG, Stelzer EHK. 2007) The third dimension bridges the gap between cell culture and live tissue, Nat Rev MCB. Colombelli J, Reynaud EG, Stelzer EHK.

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microscopy is light at work - Introduction to LSFM

Light-Sheet-based Fluorescence Microscopy (LSFM, SPIM, DSLM). Most optical technologies (microscopy, optical tweezers [1], laser nanoscalpel [2]) are applied to two-dimensional cellular systems, i.e. they are used in a cellular context that is defined by hard and flat surfaces. However, physiological meaningful information relies on the morphology, the mechanical properties, the media flux and the biochemistry of a cell’s context found in live tissue [3, 4]. Based on this outstanding performance, we clai...

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