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University of Massachusetts Medical School. Steven Reppert (sketch); Monarch Watch. Since 2002, the laboratory of Steven M. Reppert has used anatomical, cellular, molecular, electrophysiological, genetic and behavioral approaches to more fully understand the biological basis of monarch butterfly (. Migration, with a focus on the butterfly's navigational abilities and its distinctive circadian clock.

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University of Massachusetts Medical School. Steven Reppert (sketch); Monarch Watch. Since 2002, the laboratory of Steven M. Reppert has used anatomical, cellular, molecular, electrophysiological, genetic and behavioral approaches to more fully understand the biological basis of monarch butterfly (. Migration, with a focus on the butterfly's navigational abilities and its distinctive circadian clock.
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Reppert Lab: HOME | reppertlab.org Reviews

https://reppertlab.org

University of Massachusetts Medical School. Steven Reppert (sketch); Monarch Watch. Since 2002, the laboratory of Steven M. Reppert has used anatomical, cellular, molecular, electrophysiological, genetic and behavioral approaches to more fully understand the biological basis of monarch butterfly (. Migration, with a focus on the butterfly's navigational abilities and its distinctive circadian clock.

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reppertlab.org reppertlab.org
1

Reppert Lab: MONARCHBASE

http://www.reppertlab.org/monarchbase

The monarch genome portal MonarchBase can be found here.

2

Reppert Lab: MIGRANTS

http://www.reppertlab.org/migrants

In the fall, decreasing daylength, cooler temperatures, and aging milkweed (genus. The larval food plant) contribute to the environmental events that trigger the migratory generation ( Goehring and Oberhauser, 2002). Migrant flight is directional; they orient to the south/southwest using a time-compensated sun compass. What are the physiological and molecular differences between migratory and non-migratory monarchs?

3

Reppert Lab: MIGRATION

http://www.reppertlab.org/migration

Modeling the sun compass. The yearly migration of eastern North American monarch butterflies is a visually and biologically breathtaking event. Migratory monarchs are at least two generations removed from those that made the journey the previous fall. They have never been to the overwintering sites before, and have no relatives to follow on their way. There must be a genetic program underlying the butterflies' migratory behavior. We want to know what that program is, and how it works.

4

Reppert Lab: WHO WE ARE

http://www.reppertlab.org/who-we-are

STEVEN REPPERT, M.D. Higgins Family Professor of Neuroscience. SHUAI ZHAN, Ph.D. Shanghai Institutes for Biological Sciences. PATRICK GUERRA, Ph.D. AMIT SINHA, Ph.D. COLLEEN FOX, M.F.A.

5

Reppert Lab: REMIGRANTS

http://www.reppertlab.org/remigrants

In the spring, the same migrants that have overwintered in Mexico remigrate northward to the southern United States to initiate the northern leg of the migration cycle. Spring remigrants also use an antenna-dependent time-compensated sun compass to direct their northward flight ( Guerra and Reppert, 2013.

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monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Geneset

http://monarchbase.umassmed.edu/geneset.html

The latest official geneset OGS2.0, which is based on the v3 assembly, consists of 15,130 genes. Query details for monarch genes. The complete information for each monarch OGS2.0 gene can be tracked here. For a specific search, input the monarch gene ID in the correct format. MonarchBase can also list all candidates with related annotation, which includes input key words. If you are interested in the old version, we also provide access for all OGS1.0 genes. Gene ID (e.g. DPOGS208079).

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - BLAST

http://monarchbase.umassmed.edu/blast.html

Search sequence using BLAST 2.2.24. Paste sequence(s) in FASTA. Blastn: Nts as input against Nts. Blastp: Pep as input against Pep. Blastx: Nts as input against Pep. Tblastn: Pep as input against Nts. Tblastx: Nts as input against Nts. Monarch genome Assembly v3. Monarch genes OGS2.0 [PEP]. Monarch genes OGS2.0 [CDS]. Monarch genome Assembly v1. Monarch genes OGS1.0 [PEP]. Monarch genes OGS1.0 [CDS]. Monarch genome scaffolds v0. Monarch genome contigs v0. Set as below: (T, true; F, false).

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Gene Families

http://monarchbase.umassmed.edu/group.html

Query genes potentially relevant for migration. Over 1,000 OGS2.0 genes of biological interest for monarch migration ( Zhan et al., 2011. Have been manually annotated and are available for browsing. Sensory input of vision. Query monarch gene families. The systemic annotation of monarch genes was based on several well-annotated databases. Monarch genes for a specific Gene Ontology. Domain, or KEGG. Orthology group can be retrieved here. Key words can also be used to search for related families.

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Migratory profiles

http://monarchbase.umassmed.edu/profile.html

Microarray analysis of unique cDNA sequences in a monarch brain EST library has been performed ( Zhu et al., 2009. By treating migrants with a juvenile hormone (JH) analog, it was possible to isolate genes involved in sun compass-oriented flight (not affected by JH status) from those involved in other, JH-dependent aspects of the migration, like reproductive function and longevity. MonarchBase has integrated these migratory profiles to the OGS2.0 geneset. Query details for monarch ESTs. P 0001 * *.

neurocrafter.com neurocrafter.com

March, 2014 | Neurocrafter

http://neurocrafter.com/2014/03

Gallery of Completed Works. I'm Sarah Wardlaw. I like talking and thinking about science. I've always felt pulled between my interest in science and creative expression, feeling like I had to choose one over the other. While in completing my PhD in neurobiology and behavior at the University of Washington, I decided it was time to integrate my interests. We need more people telling compelling stories about science. This is my effort. Follow Me for Sketches, Doodles, and More. Date archive for: March 2014.

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - MicroRNAs

http://monarchbase.umassmed.edu/miRNA.html

Because summer and migratory butterflies share the same genome, changes in gene regulatory factors may help define the migratory states. We therefore used deep sequencing to identify and profile monarch microRNAs ( Zhan et al., 2011. MonarchBase provides the access to the normalized expression values. [HELP]. Show miRNAs up-regulated in migrants. Show miRNAs down-regulated in migrants.

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Genome

http://monarchbase.umassmed.edu/genome.html

The latest monarch genome assembly (v3) includes 5,397 scaffolds spanning 249 Mb. The N50 size of the v3 assembly is 715 Kb, and the N90 size is 160 Kb. Ninety percent of the monarch assembly is now represented by 366 of the longest scaffolds. Browse the latest genome assembly (v3). Use GBrowse to browse the genome with full annotation and features. Scaffold ID (e.g. DPSCF300282:74000.80000). Gene ID (e.g. DPOGS208079). Get the nucleotide sequence for a specific region or the whole scaffold.

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Help

http://monarchbase.umassmed.edu/help.html

Browse genome using GBrowse. Query a single gene. Search a group of genes. Query ESTs and migratory profiles. Browse expanded and contracted gene families. Use the appropriate formats when searching data with IDs:. C1 or BF010 xxxxxxx. Go back to the menu. For BLAST, use sequences as input to search for monarch scaffolds, contigs, genes, ESTs, as well as proteins of other insect orders. Select the appropriate search programs and databases. Advanced users can also set the optional parameters. Of MonarchBa...

monarchbase.umassmed.edu monarchbase.umassmed.edu

MonarchBase - Migratory profiles

http://monarchbase.umassmed.edu/variation.html

Approximately 100 monarch butterflies ( D. plexippus. From around the world, as well as nine relatives (other Danaus. Species), were sequenced with 10-20x coverage. Raw reads were mapped to the v3 assembly for SNP calling. A series of population genomic analyses were then performed to decipher the history of the monarch's evolutionary origin and global dispersal, and characterize the genes and pathways associated with migratory behavior ( Zhan et al., 2014. Check polymorphism for a designated site.

neurocrafter.com neurocrafter.com

Monarch migration. | Neurocrafter

http://neurocrafter.com/monarch-migration

Gallery of Completed Works. I'm Sarah Wardlaw. I like talking and thinking about science. I've always felt pulled between my interest in science and creative expression, feeling like I had to choose one over the other. While in completing my PhD in neurobiology and behavior at the University of Washington, I decided it was time to integrate my interests. We need more people telling compelling stories about science. This is my effort. Follow Me for Sketches, Doodles, and More. On 16 March, 2014. Your emai...

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Reppert Lab: HOME

University of Massachusetts Medical School. Steven Reppert (sketch); Monarch Watch. Since 2002, the laboratory of Steven M. Reppert has used anatomical, cellular, molecular, electrophysiological, genetic and behavioral approaches to more fully understand the biological basis of monarch butterfly (. Migration, with a focus on the butterfly's navigational abilities and its distinctive circadian clock.

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Reppert Family Genealogy Pages

We are currently going through an upgrade, please check back after March 16th 2014, for our updated website. Thank You for your patience and understanding. This site powered by The Next Generation of Genealogy Sitebuilding. Written by Darrin Lythgoe 2001-2005.